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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
11/08/2016 |
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Actualizado : |
12/03/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ROCHA, C.M.L.; VELLICCE, G.R.; GARCÍA, M.G.; PARDO, E.M.; RACEDO, J.; PERERA, M.F.; DE LUCÍA, A.; BONNECARRERE, M.; GERMAN, S.; MARCELINO, F.; LEDESMA, F.; REZNIKOV, S.; PLOPER, D.L.; WELIN, B.; CASTAGNARO, A.P. |
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Afiliación : |
CARLA MARÍA LOURDES ROCHA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; GABRIEL RICARDO VELLICCE, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; MARÍA GABRIELA GARCÍA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; ESTEBAN MARIANO PARDO, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; JOSEFINA RACEDO, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; MARÍA FRANCISCA PERERA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; ADRIÁN DE LUCÍA, INTA (Instituto Nacional de Tecnología Agropecuaria) - Cerro Azul; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVIA ELISA GERMAN FAEDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANCISMAR MARCELINO, EMBRAPA Soja; FRANCISCO LEDESMA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; SEBASTIÁN REZNIKOV, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; LEONARDO DANIEL PLOPER, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; BJORN WELIN, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; ATILIO PEDRO CASTAGNARO, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino. |
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Título : |
Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2015, v. 18, no. 6, p. 439-444. OPEN ACCESS. |
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DOI : |
10.1016/j.ejbt.2015.06.007 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 24 April 2015. Accepted 23 September 2015. Available online 28 October 2015. |
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Contenido : |
ABSTRACT.
Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919
markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies.
© 2015 Pontificia Universidad Católica de Valparaíso. Production and hosting by Elsevier B.V. All rights reserve MenosABSTRACT.
Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919
markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an impor... Presentar Todo |
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Palabras claves : |
ASIAN SOYBEAN RUST; GENETIC VARIATION; GLYCINE MAX; MOLECULAR MARKERS. |
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Thesagro : |
MARCADORES MOLECULARES; SOJA; VARIACION GENETICA. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/5850/1/Rocha-C.M.-2015.-Electr.Jr.Biotech.-v18-p.439-444.pdf
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Marc : |
LEADER 02957naa a2200397 a 4500
001 1055241
005 2019-03-12
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1016/j.ejbt.2015.06.007$2DOI
100 1 $aROCHA, C.M.L.
245 $aUse of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi.$h[electronic resource]
260 $c2015
500 $aArticle history: Received 24 April 2015. Accepted 23 September 2015. Available online 28 October 2015.
520 $aABSTRACT. Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies. © 2015 Pontificia Universidad Católica de Valparaíso. Production and hosting by Elsevier B.V. All rights reserve
650 $aMARCADORES MOLECULARES
650 $aSOJA
650 $aVARIACION GENETICA
653 $aASIAN SOYBEAN RUST
653 $aGENETIC VARIATION
653 $aGLYCINE MAX
653 $aMOLECULAR MARKERS
700 1 $aVELLICCE, G.R.
700 1 $aGARCÍA, M.G.
700 1 $aPARDO, E.M.
700 1 $aRACEDO, J.
700 1 $aPERERA, M.F.
700 1 $aDE LUCÍA, A.
700 1 $aBONNECARRERE, M.
700 1 $aGERMAN, S.
700 1 $aMARCELINO, F.
700 1 $aLEDESMA, F.
700 1 $aREZNIKOV, S.
700 1 $aPLOPER, D.L.
700 1 $aWELIN, B.
700 1 $aCASTAGNARO, A.P.
773 $tElectronic Journal of Biotechnology, 2015$gv. 18, no. 6, p. 439-444. OPEN ACCESS.
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INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
07/11/2018 |
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Actualizado : |
07/11/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
DAMIÁN, J.P.; HÖTZEL ,M.J.; BANCHERO, G.; UNGERFELD, R. |
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Afiliación : |
JUAN PABLO DAMIÁN, UdelaR (Universidad de la República), FVET ( Facultad de Veterinaria), Montevideo, Uruguay.; MARIA JOSÉ HÖTZEL, Laboratório de Etologia Aplicada e Bem-Estar Animal, Departamento de Zootecnia e Desenvolvimento Rural, Universidade Federal de Santa Catarina, Brazil.; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODOLFO UNGERFELD, UdelaR (Universidad de la República), FVET ( Facultad de Veterinaria), Montevideo, Uruguay. |
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Título : |
Growing without a mother during rearing affects the response to stressors in rams. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Applied Animal Behaviour Science, December 2018, volumen 209, pages 36-40. |
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DOI : |
10.1016/j.applanim.2018.08.022 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 18 September 2017 // Received in revised form 7 August 2018 // Accepted 31 August 2018 // Available online 05 September 2018. |
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Contenido : |
ABSTRACT.
Early maternal care contributes to the normal development of the physiological, social and emotional functions of the offspring. As sheep are a strongly gregarious species, separation and isolation of an individual from the flock provokes fear and, consequently a stress response. The first aim of this study was to compare the stress response to social isolation of rams that were reared by their mothers or artificially reared. A complementary objective was to compare the cortisol response to an ACTH challenge in both groups of rams. Rams were: 1) separated from their dams 24?36 h after birth (Week 0) and artificially fed with sheep milk until Week 10 (group AR, n = 14); or 2) reared by their dams until Week 10 (group DR, n = 13). The rams were then kept in separate, adjacent paddocks for the rest of the study. When they were 8 months old, 10 animals randomly selected from each group were isolated from the group in an unfamiliar pen during 10 min. Behavioural (the number of lines crossed, frequencies of vocalizations, eliminations, sniffing, escape attempts and freezing), physiological (heart and respiratory rate, and rectal temperature), biochemical (total blood proteins, globulins and albumin), and endocrine (serum cortisol concentrations) variables were recorded. Dam-reared rams eliminated (defecations + urinations) more times and crossed more lines during the social isolation test than AR lambs (P < 0.05). The increase in total proteins and albumin concentration after the social isolation test was also greater in DR than AR rams (P = 0.01 and P < 0.01 respectively). Artificially-reared rams had greater globulin concentration than DR rams after the test (P = 0.04). When rams were 2 years old, ACTH was administered to 10 rams randomly selected from each group, and the cortisol response was measured. Dam-reared rams had greater cortisol concentrations than AR rams at 150 and 180 min after the administration of ACTH (P < 0.05). In conclusion, artificially reared rams were less affected by social isolation and had lower adrenal response to an ACTH challenge than rams reared by their mothers. This study suggests that the relationship with the mother plays a key role in the development of rams? social behaviour. © 2018 Elsevier B.V. MenosABSTRACT.
Early maternal care contributes to the normal development of the physiological, social and emotional functions of the offspring. As sheep are a strongly gregarious species, separation and isolation of an individual from the flock provokes fear and, consequently a stress response. The first aim of this study was to compare the stress response to social isolation of rams that were reared by their mothers or artificially reared. A complementary objective was to compare the cortisol response to an ACTH challenge in both groups of rams. Rams were: 1) separated from their dams 24?36 h after birth (Week 0) and artificially fed with sheep milk until Week 10 (group AR, n = 14); or 2) reared by their dams until Week 10 (group DR, n = 13). The rams were then kept in separate, adjacent paddocks for the rest of the study. When they were 8 months old, 10 animals randomly selected from each group were isolated from the group in an unfamiliar pen during 10 min. Behavioural (the number of lines crossed, frequencies of vocalizations, eliminations, sniffing, escape attempts and freezing), physiological (heart and respiratory rate, and rectal temperature), biochemical (total blood proteins, globulins and albumin), and endocrine (serum cortisol concentrations) variables were recorded. Dam-reared rams eliminated (defecations + urinations) more times and crossed more lines during the social isolation test than AR lambs (P < 0.05). The increase in total proteins and albumin concentration ... Presentar Todo |
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Palabras claves : |
CORTISOL; MOTHER-OFFSPRING BOND; SHEEP; STRESS. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03098naa a2200229 a 4500
001 1059281
005 2018-11-07
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.applanim.2018.08.022$2DOI
100 1 $aDAMIÁN, J.P.
245 $aGrowing without a mother during rearing affects the response to stressors in rams.$h[electronic resource]
260 $c2018
500 $aArticle history: Received 18 September 2017 // Received in revised form 7 August 2018 // Accepted 31 August 2018 // Available online 05 September 2018.
520 $aABSTRACT. Early maternal care contributes to the normal development of the physiological, social and emotional functions of the offspring. As sheep are a strongly gregarious species, separation and isolation of an individual from the flock provokes fear and, consequently a stress response. The first aim of this study was to compare the stress response to social isolation of rams that were reared by their mothers or artificially reared. A complementary objective was to compare the cortisol response to an ACTH challenge in both groups of rams. Rams were: 1) separated from their dams 24?36 h after birth (Week 0) and artificially fed with sheep milk until Week 10 (group AR, n = 14); or 2) reared by their dams until Week 10 (group DR, n = 13). The rams were then kept in separate, adjacent paddocks for the rest of the study. When they were 8 months old, 10 animals randomly selected from each group were isolated from the group in an unfamiliar pen during 10 min. Behavioural (the number of lines crossed, frequencies of vocalizations, eliminations, sniffing, escape attempts and freezing), physiological (heart and respiratory rate, and rectal temperature), biochemical (total blood proteins, globulins and albumin), and endocrine (serum cortisol concentrations) variables were recorded. Dam-reared rams eliminated (defecations + urinations) more times and crossed more lines during the social isolation test than AR lambs (P < 0.05). The increase in total proteins and albumin concentration after the social isolation test was also greater in DR than AR rams (P = 0.01 and P < 0.01 respectively). Artificially-reared rams had greater globulin concentration than DR rams after the test (P = 0.04). When rams were 2 years old, ACTH was administered to 10 rams randomly selected from each group, and the cortisol response was measured. Dam-reared rams had greater cortisol concentrations than AR rams at 150 and 180 min after the administration of ACTH (P < 0.05). In conclusion, artificially reared rams were less affected by social isolation and had lower adrenal response to an ACTH challenge than rams reared by their mothers. This study suggests that the relationship with the mother plays a key role in the development of rams? social behaviour. © 2018 Elsevier B.V.
653 $aCORTISOL
653 $aMOTHER-OFFSPRING BOND
653 $aSHEEP
653 $aSTRESS
700 1 $aHÖTZEL ,M.J.
700 1 $aBANCHERO, G.
700 1 $aUNGERFELD, R.
773 $tApplied Animal Behaviour Science, December 2018, volumen 209, pages 36-40.
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